Cellulase Production by Solid-state Fermentation of Marine Aspergillus ZJUBE-1 and Its Enzymological Properties Cellulase Production by Aspergillus ZJUBE-1

Solid-state fermentation conditions were investigated for cellulase production by marine Aspergillus ZJUBE-1 using crop straw as the main material. When Aspergillus ZJUBE-1 was grown under the condition: ratio of rice straw to wheat bran was 1:1, the ratio of material to liquid was 1:2, the ratio of nitrogen and carbon was 5.6%, NaCl was 2.4~4.0%, temperature at 37 °C, the maximum CMCase activity was 4705U.g, β-Gluase (2750U.g),and FPA (245U.g ) activities were obtained after 910 days of fermentation, respectively. Enzymological properties were studied, too.The optimum reaction temperature for the three kinds enzyme was 65°C and the optimum reaction pH was 4~5. Keep 80 °C for 1 hour, CMCase still keeping 10% of the activity, 70 °C for 1 hour, β-glucosidase activity only loss 60% . Fe, Mn, Mg was activator to cellulase, but Hg, Cu, Agcan inhibition strongly to cellulase activity. Keywords-marine Aspergillus sp; solid-state fermentation; cellulase; enzymologic properties